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. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: Bone. 2017 Dec 5;107:172–180. doi: 10.1016/j.bone.2017.12.001

Figure 4.

Figure 4

Knockdown of ARHGAP18 suppresses mdMSC adipogenesis. (A) Western blots probing for adipogenic markers aP2, PPARγ, APN, and perilipin following knockdown of ARHGAP18. (B) Densitometry of radiograph bands from Western blots shows significantly decreased expression of aP2 (p<0.05, n=5), PPARγ (p<0.01, n=3), APN (p<0.01, n=5), and perilipin (p<0.05, n=3). (C, D) mdMSC cells stained with oil-red-O, with control shRNA or knockdown of ARHGAP18. (E) Western blot of APN following ARHGAP18 depletion and addition of ROCK inhibitor (Y27632, 10 μM).