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. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: Bone. 2017 Dec 5;107:172–180. doi: 10.1016/j.bone.2017.12.001

Figure 5.

Figure 5

Osteogenic potential of mdMSCs is enhanced in the absence of ARHGAP18. (A, B) Alkaline phosphatase staining of control (A) and ARHGAP18 deplete (B) MSCs (10x magnification). Light microscopy (10x lens) of control (C) and ARHGAP18 knockdown cells after 7 days in osteogenic media showing presence of mineralized nodules following ARHGAP18 knockdown (D). qPCR analysis of osteogenic genes (E) Sp7 (osterix), (F) Alpl (alkaline phosphatase), and Bglap (osteocalcin) in control and ARHGAP18 knockdown mdMSCs. *p<0.05, **p<0.01, n=3 for all assays.