On day 0, recipients received 9.2Gy TBI and donor marrow. On day 1 recipient dogs were given apheresed donor buffy coat cells obtained from their respective donors. Immunosuppression (arrows) consisted of 0.4 mg/kg MTX injected IV on days 1, 3, 6, 11, and oral CSP given on days -1 to 78 at dosing designed to maintain serum levels at 100–300 ng/ml. Oral ursodiol was given on days -1 to 80 to reduce the incidence of liver GVHD. Clinical signs of GVHD occurred from days 47 to 112 (tick marks).