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. 2017 Dec 22;8:1831. doi: 10.3389/fimmu.2017.01831

Figure 1.

Figure 1

The kinetics of the cell-mediated immune response to Mycobacterium bovis infection in the white rhinoceros. Following M. bovis infection, whole blood from three animals (PB1, PB2, and PB4) was incubated overnight at 37°C without stimulation, or stimulation with bovine PPD purified protein derivative (PPDb), avian PPD (PPDa), and 6 kDa early secretory antigenic target (ESAT-6)/10 kDa culture filtrate protein (CFP-10)/TB7.7 peptides, respectively. Plasma interferon-gamma (IFN-γ) was measured by enzyme-linked immunoassay, in duplicate, as the optical density (OD), and antigen-specific release calculated as the OD derived for the unstimulated sample subtracted from that derived for each antigen (mean ΔOD ± SD). (A) Antigen-specific IFN-γ release in response to PPDb and PPDa, and the differential response to these antigens (ΔPPDb-a); (B) Antigen-specific IFN-γ release in response to ESAT-6/CFP-10/TB7.7 peptides.