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. 2017 Dec 22;8:1831. doi: 10.3389/fimmu.2017.01831

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Copyright © 2017 Parsons, Morar-Leather, Buss, Hofmeyr, McFadyen, Rutten, van Helden, Miller and Michel.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The kinetics of the humoral response to Mycobacterium bovis infection in the white rhinoceros. Following M. bovis infection, serum antibodies of three animals (PB1, PB2, and PB4) were measured using selected commercial assays and an in-house bovine purified protein derivative (PPDb) enzyme-linked immunoassay (ELISA). (A) Serological responses to MPB83 and 6 kDa early secretory antigenic target (ESAT-6)/10 kDa culture filtrate protein (CFP-10) (Dual Path Platform (DPP)^® VetTB assay) and MPB83/70 (Bovid DPP assay); seroreactivity to ESAT-6/CFP-10/MPB83 (ElephantTB STAT-PAK^® assay) is indicated (*). (B) Serological responses to PPDb, measured in duplicate by indirect ELISA, and calculated as the difference in optical density (OD) between control wells and PPDb-coated wells (mean ΔOD ± SD).