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. 2017 Dec 22;8:2557. doi: 10.3389/fmicb.2017.02557

Figure 3.

Figure 3

ZIKV induces the secretion of inflammatory cytokines and chemokines by HBMECs. (A) HBMECs were mock-treated or infected with ZIKVPE243, with a MOI of 1. After different time points, supernatants were harvested and IL-6, IL-8, and CCL5 were measured by ELISA. Data are represented as mean ± SD of two independent experiments and *p < 0.05 in relation to mock. (B,C) HBMECs were mock-treated or infected with ZIKVPE243 or ZIKVMR766, with a MOI of 1. After 48 hpi, supernatants were harvested and IL-6 (B) and CCL5 (C) were measured by ELISA. Data are represented as mean ± SD of eight independent experiments and *p < 0.05 in relation to mock. (D,E) HBMECs were treated as in (B) and cell lysates were obtained at the indicated time points. Expression of IFN-β mRNA (D) or IFN-λ1 and IFN-λ4 mRNA (E) were measured by qRT-PCR; GAPDH expression was measured as a housepkeeping control. Bars indicate ΔΔCt values, normalized according to gapdh values and mock results. Data are represented as mean ± SD of three independent experiments. #p < 0.05 in relation to ZIKVPE243.