Figure 6.

ZIKV extravasation depends on viral replication and basolateral release or transcytosis. (A) HBMECs were cultured with chloroquine (Chlor), nystatin (Nys), or brefeldin a (BFA) for 48 h, except for BFA, which was added in the last 10 h culture. Then, cell viability was analyzed by XTT assay. Cells were also cultured with culture medium only or with triton, as negative and positive controls, respectively. (B–D) HBMECs were cultured onto transwell systems and then infected with ZIKV_PE243, from the apical side. The infected cells were treated or not with Chlor, Nys, or BFA and cultured for 48 h. (B) TEER was measured at 24 and 48 hpi, (C,D) After 48 hpi, the supernatants from the upper (luminal) (C) and lower (abluminal) (D) chambers were harvested and virus RNA was measured by qRT-PCR. The data indicate the percentage of virus RNA in relation to cells infected in the absence of inhibitors. Data represents the average of three independent experiments; ^*p < 0.05.