Table 1.
Comparison of limb regeneration stages in different salamanders
| Stage/salamander | Notophthalmus viridescens (Iten & Bryant, 1973) (dpa) | Ambystoma mexicanum (Tank et al., 1976) (dpa) | Plethodon dorsalis, P. cinereus (Scadding, 1981) (dpa) | Desmognathus ochrophaeus (Scadding, 1981) (dpa) | Eurycea bislineata (Scadding, 1981) (dpa) | Bolitoglossa ramosi (dpa) |
|---|---|---|---|---|---|---|
| Wound healing | 1−5 | 1−5 | 1−2 | 1−2 | 1−2 | 1−3 |
| Early blastema | 17 | 15 | 21 | 14 | 15 | 10 |
| Medium blastema | 20 | 20 | 24 | 21 | − | 20 |
| Pigmented blastema | − | − | − | − | − | 40 |
| Late blastema | 24 | 24 | − | − | − | 60 |
| Palette | 28 | 30 | 27 | 28 | 21 | 70 |
| Digital outgrowth | 44 | 40 | 50‐71 | 34 | 30 | 95 |
Few regenerative studies have been conducted in species of the family Plethodontidae, such as Plethodon dorsalis and P. cinereus, Desmognathus ochrophaeus, D. fuscus and Eurycea bislineata. The stage described in the present study, “pigmented blastema” (40 dpa) has not been described in other salamander species. Another notable difference between B. ramosi and model salamanders (axolotls and newts) is the time taken to reach the digital outgrowth, which is longer in B. ramosi (95 dpa).