Figure 3.

Ca²⁺- and Mg²⁺-induced changes in exposed hydrophobicity of recombinant CML15 and CML16 as demonstrated by ANS fluorescence. Fluorometric scans (430-600 nm) were recorded following the addition of 15 μM (A) CML15 or (B) CML16 to 250 μM ANS where all sample conditions used ANS buffer (10 mM Tris-Cl pH 7.5, 100 mM KCl, and 1 mM DTT). The y-axis depicts fluorescence in international arbitrary units (IAU). Fluorescence values were analyzed relative to a protein-free ANS control sample that was subtracted from the data as background. Apo-CML (CML + ANS alone) scans represent baseline CML hydrophobicity. Scans were recorded under the conditions noted in the figure panels by adding various compounds to apo-CML. Each data set is representative of five experimental replicates.