Table 1.
Thermodynamic parameters and dissociation values for Ca2+ binding to CML15 and CML16.
| Mg2+-free background, Ca2+ titration | 5 mM Mg2+ background, Ca2+ titration | |||||
|---|---|---|---|---|---|---|
| Kd (μM)a | ΔH (kJ mol−1) | ΔS (J K−1 mol−1) | Kd (μM) | ΔH (kJ mol−1) | ΔS (J K−1 mol−1) | |
| CML15 | ||||||
| Site 1 | 0.22 ± 0.06 | 7.30 ± 3.84 | 151.46 | 1.56 ± 0.62 | −9.12 ± 3.18 | −102.38 |
| Site 2 | 1.23 ± 0.16 | −60.17 ± 1.04 | −85.35 | 7.25 ± 0.51 | 48.99 ± 11.34 | 147.19 |
| CML16 | ||||||
| Site 1 | 0.03 ± 0.01 | −42.38 ± 0.83 | 5.77 | 0.91 ± 0.28 | −7.49 ± 1.98 | 90.79 |
| Site 2 | 0.21 ± 0.02 | 11.93 ± 1.05 | 167.07 | 7.09 ± 0.59 | −69.12 ± 13.13 | −129.29 |
| Site 3 | 3.76 ± 0.23 | −55.06 ± 0.82 | −77.82 | ND | ||
a
Mean ± SE values.