Table 2.
Detailed reconciled elemental and macromolecular composition of cells grown on glycerol and methanol at different growth rates, and the averaged biomass composition used for defining the stoichiometric coefficients in iMT1026 v3.0. Values represent weight/weight % ± SD
| Glycerol | Methanol | Average glycerola | Average methanola | Glucoseb | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| D (h−1) | 0.035 | 0.065 | 0.100 | 0.160 | 0.035 | 0.065 | 0.100 | |||
| Protein | 36.0 ± 6.7 | 41.2 ± 0.4 | 41.1 ± 2.9 | 43.3 ± 2.8 | 48.6 ± 1.0 | 50.7 ± 0.5 | 51.5 ± 2.1 | 41.0 ± 1.5 | 50.1 ± 0.8 | 37.0 ± 2.4 |
| Carbohydrate | 45.6 ± 7.5 | 37.0 ± 1.1 | 35.9 ± 3.1 | 33.9 ± 1.3 | 29.0 ± 2.8 | 28.9 ± 0.8 | 23.5 ± 1.3 | 35.9 ± 2.0 | 27.3 ± 1.5 | 36.9 ± 3.5 |
| Lipid | 1.3 ± 0.3 | 1.8 ± 0.4 | 2.2 ± 0.5 | 2.8 ± 3e‐2 | 2.2 ± 0.2 | 1.9 ± 0.1 | 2.4 ± 0.6 | 2.5 ± 0.4 | 2.0 ± 0.2 | 6.2 ± 3.3 |
| RNA | 6.0 ± 0.1 | 6.7 ± 1.1 | 7.6 ± 1.2 | 9.1 ± 2.6 | 5.7 ± 0.6 | 6.3 ± 1.3 | 7.1 ± 0.4 | 7.8 ± 0.6 | 7.0 ± 0.6 | 6.6 ± 0.7 |
| DNA | 0.19 ± 1e‐3 | 0.19 ± 0.01 | 0.18 ± 0.01 | 0.18 ± 0.01 | 0.19 ± 4e‐3 | 0.18 ± 4e‐3 | 0.18 ± 0.02 | 0.19 ± 0.01 | 0.18 ± 0.01 | 0.13 ± 0.01 |
| SO4 | 0.28 ± 0.06 | 0.40 ± 1e‐3 | 0.45 ± 0.10 | 0.46 ± 0.11 | 0.63 ± 4e‐3 | 0.69 ± 0.05 | 0.66 ± 0.06 | 0.46 ± 0.08 | 0.63 ± 0.04 | 0.3 ± 0.3 |
| H2O | 5.8 ± 0.6 | 5.7 ± 0.2 | 6.6 ± 2.5 | 6.6 ± 0.2 | 8.4 ± 0.1 | 6.2 ± 1.8 | 8.1 ± 0.7 | 5.6 ± 0.7 | 7.2 ± 0.9 | 6.3 ± 2.4 |
| Metals | 5.3 ± 1.0 | 7.0 ± 0.1 | 6.0 ± 0.9 | 5.8 ± 1.1 | 5.3 ± 2.1 | 5.2 ± 2.0 | 6.6 ± 0.1 | 7.0 ± 0.6 | 6.3 ± 0.6 | 6.4 ± 0.4 |
| C | 42.3 ± 0.1 | 41.9 ± 0.1 | 41.9 ± 0.9 | 42.44 ± 1.00 | 42.2 ± 0.8 | 43.2 ± 0.1 | 41.8 ± 0.2 | 41.98 ± 0.27 | 42.28 ± 0.53 | 43.0 ± 1.4 |
| H | 6.3 ± 3e‐2 | 6.24 ± 2e‐3 | 6.3 ± 0.2 | 6.44 ± 0.08 | 6.6 ± 0.1 | 6.5 ± 0.2 | 6.5 ± 0.1 | 6.24 ± 0.06 | 6.43 ± 0.05 | 6.3 ± 0.2 |
| N | 7.4 ± 1.2 | 8.4 ± 0.1 | 8.7 ± 0.7 | 9.22 ± 0.24 | 9.7 ± 0.1 | 10.2 ± 0.1 | 10.4 ± 0.4 | 8.58 ± 0.31 | 10.06 ± 0.16 | 6.9 ± 0.4 |
| O | 37.8 ± 2.2 | 35.4 ± 4e‐2 | 36.0 ± 2.6 | 34.98 ± 0.40 | 35.2 ± 1.3 | 33.9 ± 1.6 | 33.4 ± 0.7 | 35.11 ± 0.58 | 33.83 ± 0.46 | 36.4 ± 1.4 |
| S | 0.25 ± 0.06 | 0.30 ± 1e‐3 | 0.25 ± 0.04 | 0.30 ± 0.04 | 0.41 ± 0.01 | 0.4 ± 0.02 | 0.43 ± 0.03 | 0.30 ± 0.03 | 0.41 ± 0.01 | 0.2 ± 0.1 |
| Ashes | 5.9 ± 1.0 | 7.7 ± 4e‐3 | 6.8 ± 1.1 | 6.6 ± 1.3 | 5.9 ± 2.0 | 5.9 ± 1.8 | 7.4 ± 0.1 | 7.8 ± 0.6 | 7.0 ± 0.6 | 7.1 ± 0.4 |
a
Average compositions are weighted averages using 1/SD.
b
Corresponding to P. pastoris growing at D = 0.1 h−1. Data taken from Carnicer et al. (2009).