Table 2.
Bacterial strains and plasmids used in this work
| Strain or plasmid | Relevant characteristicsa | Reference |
|---|---|---|
| Strains | ||
| Escherichia coli CC118 | Cloning host; Δ(ara‐leu) araD ΔlacX174 galE galK phoA thiE1 rpsE (SpR) rpoB (RifR) argE(Am) recA1 | Grant et al. (1990) |
| Escherichia coli HB101 |
Helper strain used for conjugation; F− λ− hsdS20(rB− mB−) recA13 leuB6(Am) araC14 Δ(gpt‐proA)62 lacY1 galK2(Oc) xyl‐5 mtl‐1 thiE1 rpsL20(SmR) glnX44(AS) |
Boyer and Roulland‐Dussoix (1969) |
| Pseudomonas putida EM42 |
KT2440 derivative; Δprophage1 Δprophage4 Δprophage3 Δprophage2 ΔTn7 ΔendA‐1 ΔendA‐2 ΔhsdRMS Δflagellum ΔTn4652 |
Martínez‐García et al. (2015) |
| Plasmids | ||
| pRK600 | Helper plasmid used for conjugation; oriV(ColE1), RK2 (mob+ tra+); CmR | Kessler et al. (1992) |
| pSEVA258 | Cloning vector; oriV RSF1010); (xylS‐Pm; standard multiple cloning site; KmR | Silva‐Rocha et al. (2013) |
| pSEVA258‐recX | pSEVA258 derivative bearing X recombinase indicated in Table 2; oriV (RFS1010); cargo [xylS‐Pm → recX], KmR | This work |
a
Antibiotic markers: Sp, spectinomycin; Rif, rifampicin; Sm, streptomycin; Cm, chloramphenicol; Km, kanamycin.