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Relative fluorescence of Mycobacterium tuberculosis (dark grey) and M. smegmatis (light grey) strains harbouring integrative plasmids encoding the egfp gene downstream of the different Pptr promoter mutants (1–16). Fluorescence of the strain expressing egfp from wt Pptr was considered as 100%. For unknown reasons, we could not introduce the plasmids containing the Pptr mutations 5 and 8 in M. smegmatis.
