Skip to main content
. 2018 Jan 1;2(1):21–41. doi: 10.7150/ntno.22091

Figure 6.

Figure 6

Strategies for CTC release and single-cell analysis. (A) NanoVelcro CTC release assay based on thermal responsive polymer brushes (PIPAAm), which are covalently modified onto a silicon nanowire substrate (SiNWS). (B) Aptamer-coated NanoVelcro Chip for capturing and releasing non-small cell lung cancer (NSCLC) CTCs from blood samples. Exonuclease digestion of DNA aptamers to release CTCs from the aptamer-functionalized SiNWS. (C) Laser microdissection (LMD) technique for single circulating melanoma cell (CMC) isolation. Sanger sequencing of the individually isolated cells. One CMC showed the BRAFV600E mutation and the negative control showed no BRAFV600E mutation. (D) Layer-by-layer gelatin nanocoating for bulk CTC release by raising the temperature to 37 °C or single-cell release of CTCs with a microtip to dissolve localized regions of the nanocoating. (A) Copyright Wiley, 2012. Reproduced with permission from reference 107; (B) Copyright Wiley, 2013. Reproduced with permission from reference 110; (C) Copyright Wiley, 2013. Reproduced with permission from reference 120; (D) Copyright Wiley, 2015. Reproduced with permission from reference 122.