Figure 4.

Treatment with MR16-1 decreased in aortic root atherosclerotic lesions of both IκBNS^−/−/LDLr^−/− and LDLr^−/− mice. (A) Representative photomicrographs of sections of aortic sinus plaque from four groups after treatment of phosphate-buffered saline (PBS) or MR16-1 (2 mg) once a week during a high-fat diet consumption of 16 weeks. Adjacent sections were processed for Elastica van Gieson staining (upper panels) and H&E staining (lower panels) (bars = 500 µm). (B) Quantitative comparison of the atherosclerotic lesion in the aortic sinus. IκBNS/LDLr KO PBS: PBS-treated IκBNS^−/−/LDLr^−/− mice, LDLr KO PBS: PBS-treated LDLr^−/− mice. IκBNS/LDLr KO MR16-1: MR16-1-treated IκBNS^−/−/LDLr^−/− mice, LDLr KO MR16-1: MR16-1-treated LDLr^−/− mice. Data are expressed as mean ± SEM. *p < 0.05 and **p < 0.01. NS, not significant.