Table 1.

Antibodies used for the detection of different tropomyosin antibodies via Western blotting.

Antibody name	Peptide sequence/Immunogen	Tropomyosin isoform detected	Dilution	Dilution buffer	Detailed characterization
α/9c (5-54) mouse monoclonal hybridoma supernatant	HQLEQNRRLT NELKLALNED	Tpm1.10 and Tpm1.12	1:500	BSA	Vrhovski et al., 2003; Schevzov et al., 2011
γ/9a mouse monoclonal affinity purified ascites	CDELYAQKLKYKA ISDELDHALNDMTSI	Tpm3.3, Tpm3.5, Tpm3.8 and Tpm3.9	1:250	Skim milk	Schevzov et al., 2011
γ/9c mouse monoclonal affinity purified ascites	CERLYSQLERNRLL SNELKLTLHGL	Tpm3.4 and Tpm3.7	1:250	Skim milk	Schevzov et al., 2011
γ/9c sheep polyclonal affinity purified ascites	CERLYSQLERNRLL SNELKLTLHGL	Tpm3.4 and Tpm3.7	1:250	Skim milk	Schevzov et al., 2011
γ/9d (2G10.2) mouse hybridoma supernatant	DKLKCTKEEHLCTQRMLD QTLLDLNEM	Tpm3.1 and Tpm3.2	1:500	Skim milk	Schevzov et al., 2011
γ/9d sheep polyclonal affinity purified ascites	DKLKCTKEEHLCTQRM LDQTLLDLNEM	Tpm3.1 and Tpm3.2	1:500	Skim milk	Schevzov et al., 2005, 2011
δ9d (WD4/9d) Tm4 rabbit affinity purified ascites	KEENVGLHQTLDTLN	Tpm4.2	1:250	BSA	Hannan et al., 1998; Schevzov et al., 2005, 2011

Detailed characterization of antibodies used in this study has previously been described by (Schevzov et al., 2011), including lack of cross reactivity of antibodies for other tropomyosin antibodies.