Table 2. qRT-PCR primer design for experiments.

Gene	PCR primer
	Length (bp)	Location
ACTB	63	Chromosome 7: exon 2–3
GAPDH	58	Chromosome 12: exon 9
ESR-1_S*	62	Chromosome 6: exon 3–4
ESR-1_L†	98	Chromosome 6: exon 1–2

qRT-PCR=quantitative real-time polymerase chain reaction; bp=base pair.

^*Short PCR primer for ESR-1 gene; ^†Long PCR primer for ESR-1 gene.