Table 6. Comparison of RNA quality of FFPE samples between center A and center B using qRT-PCR.
Center | RNA quality | ACTB | GAPDH | ESR-1 |
---|---|---|---|---|
Center A (n = 50) | Success rate, % (n) | 84.0 (42/50) | 88.0 (44/50) | 78.0 (39/50) |
Average CT | 32.23 | 32.21 | 33.76 | |
Center B (n = 46) | Success rate, % (n) | 41.3 (19/46) | 47.8 (22/46) | 41.3 (19/46) |
Average CT | 36.55 | 36.91 | 36.02 |
FFPE=formalin-fixed paraffin-embedded; qRT-PCR=quantitative real-time polymerase chain reaction; CT=threshold cycle.