Table 7. Comparison of RNA quality of FFPE samples between center A and center B using NGS.
Center | RNA quality | ACTB | GAPDH | ESR-1 |
---|---|---|---|---|
Center A (n = 50) | Success rate, % (n) | 100 (50/50) | 100 (50/50) | 100 (50/50) |
Average FPKM | 31,313.31 | 16,300.13 | 4,802.48 | |
Center B (n = 46) | Success rate, % (n) | 93.5 (43/46) | 93.5 (43/46) | 93.5 (43/46) |
Average FPKM | 51,369.42 | 6,114.09 | 5,569.75 |
FFPE=formalin-fixed paraffin-embedded; NGS=next-generation sequencing; FPKM=fragments per kilobase of exon per million fragments mapped.