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. 2017 Dec 22;4:232. doi: 10.3389/fvets.2017.00232

Figure 1.

Figure 1

Receiver operating characteristic (ROC) curves of quantitative results of quantitative PCR (qPCR) versus reference tests; tissue culture (A–C) or histopathology (D–F). (A) ROC curve of qPCR of intestine against culture results as reference test. Area under the curve (AUC) was 0.88 [95% confidence interval (CI): 0.82, 0.95], and at a cutoff of >0.00054 pg, the sensitivity was 88.33% (95% CI: 77.43%, 95.18%), and the likelihood ratio was 4.16. (B) ROC curve of qPCR of mesenteric lymph node (MLN) against culture results as reference test. AUC was 0.85 (95% CI: 0.77, 0.92), and at a cutoff of >0.00052 pg, the sensitivity was 86.44% (95% CI: 75.02%, 93.96%), and the likelihood ratio was 2.19. (C) ROC curve of qPCR of intestine and MLN against culture results of both tissues as reference test. AUC was 0.85 (95% CI: 0.78, 0.92), and at a cutoff of >0.00053 pg, the sensitivity was 95.31% (95% CI: 86.91%, 99.02%), and the likelihood ratio was 2.28. (D) ROC curve of qPCR of intestine against histopathological lesion examination as reference test. AUC was 0.89 (95% CI: 0.82, 0.96), and at a cutoff of >0.00054 pg, the sensitivity was 85.71% (95% CI: 72.76%, 94.06%), and the likelihood ratio was 2.68. (E) ROC curve of qPCR of MLN against histopathological lesion examination as reference test. AUC was 0.81 (95% CI: 0.72, 0.89), and at a cutoff of >0.00052 pg, the sensitivity was 81.82% (95% CI: 69.10%, 90.92%), and the likelihood ratio was 1.99. (F) ROC curve of qPCR of intestine and MLN against histopathological lesion examination of both tissues as reference test. AUC was 0.84 (95% CI: 0.77, 0.92), and at a cutoff of >0.00053 pg, the sensitivity was 89.66% (95% CI: 78.83%, 96.11%), and the likelihood ratio was 1.80.