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. 2017 Dec 22;4:232. doi: 10.3389/fvets.2017.00232

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Copyright © 2017 Acharya, Dhand, Whittington and Plain.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Scatter plot of mean log₁₀ DNA quantified by mesenteric lymph node (MLN) quantitative PCR (qPCR) against log₁₀ DNA quantified by intestinal tissue (gut) qPCR. The solid line is the regression line. A positive correlation coefficient of 0.78 (95% CI: 0.69, 0.84) was observed. (B) Bland–Altman plot depicting difference of log₁₀ DNA quantified by gut qPCR and MLN qPCR against average log₁₀ DNA quantified by them. The dotted line shows the line of perfect agreement whereas two solid lines show the limit of agreement. The DNA quantified by gut qPCR was 2.94 times higher than that quantified by MLN qPCR for the same animal on an average. The solid line is the regression line of average log₁₀ DNA against difference of log₁₀ DNA. An increase in average difference was observed for the increase in DNA quantity in the gut and MLN homogenate.