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. 2017 Dec 22;8:1922. doi: 10.3389/fimmu.2017.01922

Figure 1.

Figure 1

Nuclear factor (erythroid-derived 2)-like 2 (Nrf2) signaling alters dendritic cell (DC) activation status. (A) Frequency of CD11c+ cells after generating (d0) or just activating (d8) bone marrow-derived DC (BMDC) in the presence of 200 µM metabolite monomethyl fumarate (MMF) (data pooled from three to five experiments). (B) Expression of CD80, CD86, and MHCII on cultured CD11c+ BMDC after MMF treatment as assessed by flow cytometry (data pooled from three to four experiments, *p < 0.05, **p < 0.01). (C) Frequency of CD11c+ cells after generating wild-type (wt), Nrf2KO, and CD11c-Cre/Nrf2-fl/fl BMDC in the presence of 200 µM MMF (data pooled from four independent experiments). (D) Frequency of CD80+CD86+ cells in CD11c+ BMDC generated from wt, Nrf2KO, and CD11c-Cre/Nrf2-fl/fl mice under MMF treatment as assessed by flow cytometry (data pooled from four experiments, *p < 0.05, **p < 0.01, ***p < 0.001). BMDC were stimulated with 1 µg/ml LPS for 48 h before analysis. LPS stimulated, but otherwise treatment naive BMDC obtained from wt mice served as control.