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. 2017 Dec 22;8:1922. doi: 10.3389/fimmu.2017.01922

Figure 2.

Figure 2

Nuclear factor (erythroid-derived 2)-like 2 (Nrf2) affects functionality of myeloid dendritic cell(DC). (A) Migratory capacity of Nrf2KO and metabolite monomethyl fumarate (MMF)-treated wild-type (wt) bone marrow-derived DC (BMDC) compared to control cells as assessed by an in vitro FCS-gradient Transwell assay (data pooled from two to five independent experiments, **p < 0.01, ***p < 0.001). (B) Phagocytic capacity of MMF versus control-treated wt and Nrf2KO BMDC determined as frequency of fluorescent bead uptake by flow cytometry (data pooled from two to four experiments, **p < 0.01, ***p < 0.001). (C–J) Gene expression analysis of nfe2l2 (Nrf2) (C), nqo1 (D), hmox1 (E), il10 (F), tgfb (G), arg1 (H), il6 (I), and il12a (J) in MMF-treated wt and Nrf2-deficient BMDC compared to control cells (data pooled from two to three preparations, *p < 0.05, **p < 0.01, ***p < 0.001). (K–N) Interleukin (IL)-6 (K), IL-12 (L), IL-23 (M), and tumor necrosis factor alpha (N) production in MMF-treated wt and Nrf2-deficient cultured BMDC (n = 6 for wt and n = 3 for CD11c-Cre/Nrf2-fl/fl, *p < 0.05, **p < 0.01, ***p < 0.001). (O) Representative immunoblot of NAD(P)H: quinone oxidoreductase 1 (Nqo1) protein expression in wt and Nrf2-deficient BMDC cultured with and without MMF. β-Actin was used as loading control.