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. 2017 Dec 22;8:1922. doi: 10.3389/fimmu.2017.01922

Figure 3.

Figure 3

Nuclear factor (erythroid-derived 2)-like 2 (Nrf2Nrf2) shows direct and indirect effects on T cell differentiation. (A–C) MOG-loaded, metabolite monomethyl fumarate (MMF) pretreated or untreated wt and Nrf2KO bone marrow-derived dendritic cell (BMDC) were cocultured with MOG-specific transgenic naive CD4+ T cells under Th1 (A), Th17 (B), or Treg (C) cell polarizing conditions (data pooled from three to six experiments, *p < 0.05, **p < 0.01). (D) Proliferation rate of T cells cultured with Nrf2KO or MMF pretreated or untreated wild-type (wt) BMDC as assessed by thymidine incorporation assay (one out of three representative experiments is shown, ***p < 0.001, cpm = counts per minute). (E–G) Addition of 200 µM MMF to wt and Nrf2KO CD4+ T cell differentiation culture under Th1 (E), Th17 (F), or Treg (G) polarizing conditions (data pooled from three to five experiments, **p < 0.01).