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. 2017 Dec 22;10:434. doi: 10.3389/fnmol.2017.00434

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Copyright © 2017 Adotevi and Leitch.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Subcellular fractionation and western blot of fractions. (A) Schematic flowchart representation of the biochemical fractionation procedure. TxS is the supernatant fraction containing Triton X-100 soluble extrasynaptic membrane, and TxP is the pellet fraction containing the Triton X-100 insoluble synaptic membrane. (B) Representative western blot of subcellular fractions of the somatosensory cortex. Images showing bands corresponding to control markers; pan-cadherin, PSD95, CaMKII and β-actin in the different fractions.