Figure 1.

Schematic diagram of zebrafish xenograft model. Colorectal cancer (CRC) tumor cells were labeled with 1,1′-dioctadecyl-3,3,3′3′-tetramethylindocarbocyanine (DiI) dye in vitro, and approximately 300 tumor cells are injected into the yolk sac of each two days post-fertilization zebrafish larvae. Tumor invasion, dissemination, metastasis, and angiogenesis can be visualized, and anti-cancer drug screening can be conducted in vivo in a matter of days.