Table 2.
The advantages and limitations in the inflammatory bowel disease model of zebrafish.
| Items | Larvae | Adult Zebrafish |
|---|---|---|
| Advantages | Many individuals | Less skin damage |
| Live imaging | Adaptive immune involved | |
| Germ-free derivation | ||
| Colonization with specific bacteria | ||
| High-throughput drug screening | ||
| Easy operation | ||
| Limitations | Chemically-induced skin damage | With craft |
| Less sample number | ||
| Example | TNBS: immersing larvae in 25–75 ug/mL TNBS from 3 dpf | OXO: 0.2% oxazolone by intrarectally injection |
| DSS: immersing larvae 0.5% (w/v) DSS from 3dpf | TNBS: 1 uL per 0.1g of body weight by intrarectally injection |
TNBS: 2,4,6-trinitrobenzene sulfonic acid; DSS: Dextran sodium sulfate; OXO: oxazolone.