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. 2017 Nov 23;9(12):355. doi: 10.3390/v9120355

Figure 4.

Figure 4

The DLG1-Tax complexes do not co-localize in endosomes or autophagosomes. (A and B) Evaluation of co-localization between the DLG1-Tax complexes and endosomal markers. HEK293 cells were transiently transfected with pcDNA-HA-DLG1 (HA, human influenza hemagglutinin epitope) and pmTurq2-Tax plasmids, along with a pgfp-RAB5 (A) or pgfp-RAB7 (B). The HA-DLG1 expression was detected by immunofluorescence using an anti-HA antibody. The DLG1-Tax co-localization pattern is shown in yellow and RAB expression is shown in blue; (C) Assessment of co-localization between the DLG1-Tax complexes and the autophagosome marker LC3. The prfp-LC3 plasmid was transfected in HEK293 cells along with both pmTurq2-Tax and pegfp-DLG1 plasmids. Each protein expression was observed by direct emission of the tagging fluorescent proteins. The DLG1-Tax colocalization pattern is shown in yellow and LC3 expression is shown in blue. In (A–C) the white arrow indicates the region magnified in the inset. All scale bars represent 10 µm.