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. 2017 Nov 23;9(12):355. doi: 10.3390/v9120355

Figure 5.

Figure 5

The DLG1-Tax complexes co-distribute with the Golgi apparatus. (A) Evaluation of co-localization of the perinuclear DLG1-Tax complexes with GM130 in overexpression experiments. HEK293 cells transiently transfected with pcDNA-HA-DLG1 and pmTurq2-Tax plasmids were immunostained to detect GM130 expression using a monoclonal anti-GM130 antibody (blue). HA-DLG1 expression was detected using an anti-HA antibody (red) and mTurq2-Tax expression was observed directly through the mTurq2 emission (green). Dashed circles indicate nuclear regions. The DLG1-Tax complexes are shown in yellow; (B) Analysis of co-localization of the endogenous DLG1-Tax complexes with GM130. HEK293 cells were transiently transfected with mTurq2-Tax (green), and endogenous DLG1 (red) and GM130 (Blue) were detected using specific antibodies. In (A,B), the light blue arrow indicates the region which is amplified in the inset, indicating the DLG1-Tax-GM130 triple co-distribution. All scale bars are 10 µm.