TABLE 1.

ResistancePlus MG kit detection of M. genitalium and 23S rRNA gene mutations with the ABI 7500 qPCR platform^a

PCR platform	Test result	No. of samples with M. genitalium 16S qPCR reference test result:		No. of samples with 23S rRNA mutation Sanger sequencing reference test resultb:
		+ve	−ve	Mutation +vec	Mutation −ve
SpeeDx ABI 7500	+ve	99	0	62	0
	−ve	2d	81	5	30
Total		101	81	67	30

^a+ve, positive; −ve, negative.

^bAnalysis of 97 M. genitalium-positive samples. Sequencing results for two samples were unavailable due to a failed sequencing run.

^cMutations (n = 67) included A2058G (n = 23), A2058T (n = 4), and A2059G (n = 40).

^dFewer than 10 genomes/5 μl of extract were detected in the samples when discrepant analysis was performed by repeating the in-house 16S qPCR.