FIG 3.

Alignment of 18S rRNA sequences for P. falciparum (Pf) and P. malariae (Pm). The PCR products from the four 18S RNA alleles predicted from the P. falciparum genome were aligned with the 18S RNA product from P. malariae obtained from control plasmid sequences, using Geneious 9.1.5 (24). The total product sizes are between 214 and 225 bp. The level of identity between P. falciparum chromosomes 1 and 13 is 97.8% (estimated melting temperature is 71.9°C), that between P. falciparum chromosomes 5 and 7 is 100% (estimated melting temperature is 73.4°C), that between P. falciparum chromosome 1 and 13 and P. malariae is 75.4%, and that between P. falciparum chromosomes 5 and 7 and P. malariae is 72.3%. The estimated P. malariae melting temperature is 71.8°C. Discordant bases are highlighted.