TABLE 1.
Microscopy versus smear and pellet PCR-HRM results for the diagnosis of malaria species in 367 patient samples from southwestern Uganda
| Sample type and PCR result | No. with microscopy result of: |
Sensitivity (95% CI) (%) | Specificity (95% CI) (%) | |
|---|---|---|---|---|
| Positive | Negative | |||
| Any malaria species | ||||
| Smear | ||||
| Positive | 172 | 6 | 93.0 (88.2–96.2) | 96.7 (93.0–98.8) |
| Negative | 13 | 176 | ||
| Pellet | ||||
| Positive | 185 | 11 | 100.0 (98.0–100.0) | 94.0 (89.4–96.9) |
| Negative | 0 | 171 | ||
| Plasmodium falciparum | ||||
| Smear | ||||
| Positive | 161 | 4 | 88.5 (82.9–92.7) | 97.8 (94.6–99.4) |
| Negative | 21 | 181 | ||
| Pellet | ||||
| Positive | 165 | 0 | 91.71 (86.6–95.2) | 100.0 (98.0–100.0) |
| Negative | 15 | 187 | ||
| Plasmodium malariae | ||||
| Smear | ||||
| Positive | 1 | 4 | 50.0 (1.2–98.7) | 98.9 (97.2–99.7) |
| Negative | 1 | 361 | ||
| Pellet | ||||
| Positive | 1 | 4 | 50.0 (1.2–98.7) | 98.9 (97.2–99.7) |
| Negative | 1 | 361 | ||
| Plasmodium ovale | ||||
| Smear | ||||
| Positive | 4 | 4 | 100.0 (39.8–100.0) | 98.9 (97.2–99.7) |
| Negative | 0 | 359 | ||
| Pellet | ||||
| Positive | 4 | 7 | 100.0 (39.8–100.0) | 98.1 (96.1–99.2) |
| Negative | 0 | 356 | ||