TABLE 1.
Positive and negative agreement of seven treponemal assays with a consensus of the test panel
| Assay and resulta | Test panel consensus (no. of samples)b |
% agreement (95% CI) |
κ value | ||
|---|---|---|---|---|---|
| Positive | Negative | Positive | Negative | ||
| BioPlex MBIA | |||||
| Positive | 870 | 16 | 95.8 (94.3–97.0) | 98.1 (97.1–98.8) | 0.95 |
| Negative | 31 | 1,066 | |||
| Indeterminate | 7 | 5 | |||
| Centaur CIA | |||||
| Positive | 905 | 43 | 99.7 (99.0–99.9) | 96.0 (94.7–97.1) | 0.95 |
| Negative | 2 | 1,044 | |||
| Indeterminate | 1 | 0 | |||
| FTA-ABS | |||||
| Positive | 782 | 8 | 86.1 (83.7–88.2) | 99.0 (98.2–99.5) | 0.91 |
| Negative | 82 | 1,076 | |||
| Indeterminate | 44 | 3 | |||
| Inno-LIA | |||||
| Positive | 841 | 16 | 92.6 (90.7–94.2) | 98.5 (97.6–99.1) | 0.92 |
| Negative | 67 | 1,071 | |||
| Liaison CIA | |||||
| Positive | 879 | 39 | 96.8 (95.4–97.8) | 96.0 (94.6–97.0) | 0.94 |
| Negative | 20 | 1,043 | |||
| Indeterminate | 9 | 5 | |||
| TP-PA | |||||
| Positive | 756 | 0 | 83.3 (80.7–85.6) | 100 (99.6–100) | 0.86 |
| Negative | 137 | 1,087 | |||
| Indeterminate | 15 | 0 | |||
| Trep-Sure EIA | |||||
| Positive | 902 | 149 | 99.3 (98.5–99.7) | 86.3 (84.1–88.2) | 0.85 |
| Negative | 5 | 938 | |||
| Indeterminate | 1 | 0 | |||
a
MBIA, microbead immunoassay; EIA, enzyme immunoassay; LIA, line immunoassay; TP-PA, Treponema pallidum particle agglutination assay; FTA-ABS, fluorescent treponemal antibody absorbed test; CIA, chemiluminescence immunoassay.
b
Consensus was defined as ≥4/7 tests positive or ≥4/7 tests negative (n = 1,995).