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. 2017 Dec 27;10:311. doi: 10.1186/s13068-017-0998-3

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Carbohydrate and gene expression analyses in OXPdDUF231A transgenic lines. Two independent OXPdDUF231A transgenic lines (OXPdDUF231A-1 and OXPdDUF231A-2), WV94 (wild type) and K-147 (empty vector control) were grown under greenhouse conditions. a Monosaccharide composition analysis. The monosaccharide composition was determined by ion chromatography after a two-step acid treatment. b Cellulose content analysis by anthrone dye staining. c Relative gene expression of cellulose biosynthesis-related genes in leaf and xylem. Shown are the mean value ± SD of three technical repeats of three biological replicates of leaf or xylem (n = 9). Asterisks indicate statistical significance compared to WV94 (p < 0.01)