Table 2.
List of metabolites with decreased content (µg/g FW sorbitol equivalents) in leaf tissues of 6-month-old greenhouse-grown OXPdDUF231A versus K-147 plants
| Metabolite [retention time (min); key m/z] | OXPdDUF231A/K-147 | OXPdDUF231A | K-147 | |||
|---|---|---|---|---|---|---|
| Fold change | p value | Avg | sem | Avg | sem | |
| Iminodiacetic acid | 0.02 | 0.003 | 3.3 | 0.9 | 188.2 | 63.6 |
| Asparagine | 0.03 | 0.000 | 2.4 | 1.0 | 68.5 | 14.7 |
| 10.84 158 200 302 | 0.03 | 0.000 | 0.6 | 0.1 | 21.2 | 2.2 |
| Lysine | 0.03 | 0.003 | 2.3 | 1.0 | 90.7 | 29.9 |
| Ornithine | 0.04 | 0.000 | 50.1 | 35.3 | 1290.1 | 170.1 |
| Rutin | 0.04 | 0.000 | 0.5 | 0.4 | 12.8 | 2.6 |
| Luteolin | 0.05 | 0.004 | 0.6 | 0.1 | 11.4 | 3.9 |
| 17.80 501 559 471 Flavonoid | 0.06 | 0.005 | 1.4 | 0.3 | 21.2 | 7.4 |
| 7.92 218 231 | 0.06 | 0.000 | 0.3 | 0.1 | 4.7 | 0.6 |
| 13.75 219 Coumaroyl glycoside | 0.07 | 0.001 | 1.3 | 0.1 | 17.6 | 4.3 |
| 9.49 302 316 288 242 208 | 0.07 | 0.000 | 4.5 | 1.7 | 68.5 | 11.6 |
| α-Keto-glutaric acid | 0.08 | 0.000 | 9.7 | 2.3 | 114.7 | 14.5 |
| 1,6-Anhydroglucose | 0.08 | 0.000 | 16.7 | 3.0 | 209.7 | 37.7 |
| Oxalomalic acid | 0.08 | 0.000 | 7.5 | 1.3 | 96.6 | 17.6 |
| Glutamine | 0.08 | 0.001 | 6.8 | 1.8 | 90.3 | 24.4 |
| 14.11 521 171 289 Glycoside | 0.09 | 0.013 | 1.9 | 0.7 | 20.0 | 8.2 |
| 3-Amino-2-piperidone | 0.09 | 0.007 | 3.7 | 3.1 | 39.8 | 12.9 |
| 10.58 315 330 200 172 | 0.09 | 0.000 | 1.3 | 0.9 | 14.3 | 2.0 |
| 10.15 368 353 271 242 184 | 0.09 | 0.000 | 6.4 | 2.4 | 71.1 | 10.9 |
| 8.99 242 257 99 | 0.10 | 0.003 | 2.2 | 0.7 | 22.0 | 6.7 |
Metabolites were analyzed as trimethylsilyl derivatives by gas chromatography–mass spectrometry. Data are the average (avg) and standard error of the mean (sem) of six OXPdDUF231A plants (three plants from each of two independent transgenic lines) and three K-147 control plants. Unknown and partially identified metabolites are designated by retention time, key mass-to-charge (m/z) ratios, and identified moieties