FIGURE 3.

The presence of the virus affects the sites of CP–CP homotypic interactions. CP–CP homotypic interactions detected by bimolecular fluorescent complementation (BiFC) assay. N. benthamiana leaves were co-infiltrated with A. tumefaciens carrying constructs to express CP-YFPn (CP fused to the N-terminal half of the YFP) and CP-YFPc (CP fused to the C-terminal part of the YFP) (A). In (B), CP-YFPn and CP-YFPc were co-expressed with a TYLCV infectious clone or mock control. Samples were observed under the confocal microscope 2 days after infiltration; CP–CP interactions are detected as yellow fluorescence. This experiment was repeated three times; more than 15 cells were observed per sample and replicate. BF, Bright field. Scale bar: 10 μm.