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. 2017 Dec 22;8:2165. doi: 10.3389/fpls.2017.02165

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Copyright © 2017 Wang, Tan, Wu, Jimenez-Gongora, Tan and Lozano-Duran.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The CP-containing nuclear foci induced by TYLCV partially co-localize with markers of sites of RNA processing. N. benthamiana leaves were co-infiltrated with A. tumefaciens carrying constructs to express CP-GFP (A) or CP-RFP (B) together with marker proteins of subnuclear compartments: coilin and fibrillarin fused to RFP (A), and ALY4, U3-55K, and RNPS1 fused to GFP (B). (For details about these marker proteins, see the “Results” section). Samples were observed under the confocal microscope 2 days after infiltration using sequential scanning. This experiment was repeated three times; more than 20 cells were observed per sample and replicate. Scale bar: 10 μm.