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. 2017 Dec 22;8:2577. doi: 10.3389/fmicb.2017.02577

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Copyright © 2017 Zhu, Chen, Li and Wen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of deletion and overexpression of avaR1 on avermectin production and cell growth in S. avermitilis. (A) Comparison of avermectin production in WT, avaR1 deletion mutant (ΔavaR1), complemented strain (CavaR1), and overexpression strain (OavaR1) cultured in FM-I for 10 days. WT/pKC1139 and WT/pSET152: vector control strains. Error bars: standard deviation (SD) from three replicate experiments. NS, not significant; ^*, P < 0.05; ^***, P < 0.001 for comparison with WT (Student's t-test). (B) Growth curves of WT, ΔavaR1, and CavaR1 in FM-II. Error bars: SD from three replicates.