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. 2017 Dec 22;8:2577. doi: 10.3389/fmicb.2017.02577

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Copyright © 2017 Zhu, Chen, Li and Wen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Relative affinities of AvaR1 for various target promoters. (A) EMSA of His₆-AvaR1 with labeled probe aveRp and unlabeled probes (aveRp, avaR2p, avaR3p, acop, avaR1p). (B) EMSA of His₆-AvaR1 with labeled probe avaR2p and unlabeled probes. (C) EMSA of His₆-AvaR1 with labeled probe avaR3p and unlabeled probes. (D) EMSA of His₆-AvaR1 with labeled probe acop and unlabeled probes. (E) EMSA of His₆-AvaR1 with labeled probe avaR1p and unlabeled probes. For competition assays, labeled probe (0.3 nM) and unlabeled competitor probe (50- and 250-fold) were added with His₆-AvaR1 (50 nM). Arrowheads: free labeled probes.