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. 2017 Dec 22;8:2577. doi: 10.3389/fmicb.2017.02577

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Copyright © 2017 Zhu, Chen, Li and Wen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Relationships between AvaR1 and AvaR2. (A) Competitive EMSAs of probe aveRp₂ with His₆-AvaR1 and GST-AvaR2 proteins. 0.3 nM labeled probe aveRp₂ was incubated with the indicated concentrations of His₆-AvaR1 and GST-AvaR2. (B) GST pull-down assays of AvaR1 and AvaR2 from E. coli whole cell lysate. His₆- and GST-tagged proteins were co-expressed in E. coli, recovered by sonication and centrifugation, and subjected to GST pull-down and Western blotting analysis with anti-GST and anti-His antibodies, respectively. Lanes 1: cell lysate before induction by IPTG. Lanes 2: cell lysate after induction. Lanes 3: GST pull-down.