Table 1.
Strains and plasmids used in this study.
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| S. avermitilis | ||
| ATCC31267 | Wild-type (WT) strain | Laboratory stock |
| ΔavaR1 | avaR1 deletion mutant | This study |
| CavaR1 | avaR1 complemented strain | This study |
| OavaR1 | avaR1 overexpression strain | This study |
| ΔavaR1/avaR1-3FLAG | avaR1 complemented strain with AvaR1-3FLAG | This study |
| WT/pKC1139 | WT strain containing control vector pKC1139 | This study |
| WT/pSET152 | WT strain containing control vector pSET152 | This study |
| ΔavaR2 | avaR2 deletion mutant | Zhu et al., 2016 |
| ΔavaR1R2 | avaR1 avaR2 double deletion mutant | This study |
| E. coli | ||
| JM109 | Routine cloning host and host for lux reporter system | Laboratory stock |
| ET12567 | Non-methylating strain | MacNeil and Klapko, 1987 |
| BL21 (DE3) | Protein overexpression host | Novagen |
| Plasmids | ||
| pKC1139 | Multiple-copy, temperature-sensitive E. coli-Streptomyces shuttle vector | Bierman et al., 1992 |
| pSET152 | Integrative E. coli-Streptomyces shuttle vector | Bierman et al., 1992 |
| pET-28a (+) | His6-tagged protein expression vector | Novagen |
| pGEX-4T-1 | GST-tagged protein expression vector | GE Healthcare |
| pJL117 | Vector carrying ermE*p (Streptomyces strong constitutive promoter) | Li et al., 2010 |
| pΔavaR1 | avaR1 deletion vector based on pKC1139 | This study |
| pKC1139-ermp-avaR1 | avaR1 overexpression vector based on pKC1139 | This study |
| pSET152-avaR1 | avaR1 complemented vector based on pSET152 | This study |
| pET28-avaR1 | His6-AvaR1 expression vector based on pET-28a (+) | Zhu et al., 2016 |
| pIJ10500 | Vector carrying 3×flag fragment | Pullan et al., 2011 |
| pSET152-avaR1-3FLAG | avaR1 complemented vector with avaR1-3×flag on pSET152 | This study |
| pCS26-Pac | Vector carrying promoterless lux reporter | Tahlan et al., 2007 |
| pOaveRlux | pCS26-Pac carrying aveR promoter-controlled lux reporter | Zhu et al., 2016 |
| pACYC184 | Protein expression vector in reporter system | Tahlan et al., 2007 |
| pAvaR1 | AvaR1 expression vector in reporter system | This study |
| pGEX-avaR2 | GST-AvaR2 expression vector based on pGEX-4T-1 | This study |
| pET28-avaR2 | His6-AvaR2 expression vector based on pET-28a (+) | Zhu et al., 2016 |