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. 2017 Dec 22;8:1097. doi: 10.3389/fphys.2017.01097

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Copyright © 2017 Bernabò, Valbonetti, Greco, Capacchietti, Ramal Sanchez, Palestini, Botto, Mattioli and Barboni.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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FRAP analysis of spermatozoa stained with DilC12 incubated with or without Aminopurvalanol A. (A) gallery showing the FRAP analysis method and the measurement of fluorescence recovery by confocal microscopy. (B) Effect of different times of incubation and Aminopurvalanol A concentrations on DilC12 diffusion coefficient. The data are reported as median and 25° and 75° percentile. We found that the data at 2 h incubation were significantly different (p < 0.01) from those at T0, while the different treatments did not exerted any significative effect (p > 0.05). The comparison were carried out with Kruskall-Wallys test.