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. 2017 Dec 22;8:2608. doi: 10.3389/fmicb.2017.02608

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Copyright © 2017 Flórez and Mayo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Southern blot analysis of total genomic DNA undigested and digested with either HindIII and PstI from the S. thermophilus strains resistant to tetracycline (A) and erythromycin (B), respectively. As a probe, internal segments of tet(S) (in A) and ermB (in B) genes obtained by specific PCR and labeled with digoxigenin (Roche) were used. Molecular weight markers: A, digoxigenin-labeled, EcoRI and HindIII-digested lambda DNA; B, digoxigenin-labeled, HindIII-digested lambda DNA. Size of the fragments (in kbp) is indicated. The code numbers of the resistant strains are given above the lane numbers.

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