Table 1.

Metabolite formulation for soil defined medium 1 (SDM1).

Metabolite amendment	Formula	Molecular weight (g/mol)	Metabolite Class	WEOC mg/L	WEOC C ppm	SDM1 mg/L	SDM1 C ppm
Trehalose	C12H22O11	342.3	dihexose	12.53	5.28	20	8.4
Fructose	C6H12O6	180.2	hexose	6.70	2.68	15	5.81
Glucose	C6H12O6	182.2	hexose	12.55	4.96	15	5.93
Mannitol	C6H14O6	182.2	sugar alcohol	8.17	3.23	15	5.93
Maltose	C12H22O11	342.3	dihexose	2.95	1.24	5	2.11
Alanine	C3H7NO2	89.1	amino acid	0.93	0.37	2	0.81
Arabinose	C5H10O5	150.1	pentose	0.52	0.21	2	0.8
Leucine	C6H13NO2	131.2	amino acid	0.7	0.38	1	0.55
Mannose	C6H12O6	180.2	hexose	0.54	0.21	1	0.4
Isoleucine	C6H13NO2	131.2	amino acid	0.46	0.25	1	0.55
Arginine	C6H14N4O2	174.2	amino acid	0.22	0.09	0.5	0.21
Proline	C5H9NO2	115.1	amino acid	0.2	0.1	0.5	0.26
Threonine	C4H9NO3	119.1	amino acid	0.2	0.08	0.5	0.2
Lysine	C6H14N2O2	146.2	amino acid	0.2	0.1	0.5	0.25
Phenylalanine	C9H11NO2	165.2	amino acid	0.19	0.13	0.5	0.33
Glycine	C2H5NO2	75.1	amino acid	0.17	0.05	0.5	0.16
Uridine	C9H12N2O6	244.2	nucleoside	0.16	0.07	0.5	0.22
Glutamate	C5H9NO4	147.1	amino acid	0.11	0.04	0.1	0.04
Serine	C3H7NO3	105.1	amino acid	0.09	0.03	0.1	0.03
Adenosine	C10H13N5O4	267.2	nucleoside	0.08	0.04	0.1	0.04
Gamma-guanidinobutyric acid	C5H11N3O2	145.2	amino acid derivative	0.05	0.02	0.1	0.04
Hypoxanthine	C5H4N4O	136.1	purine	0.04	0.02	0.1	0.04
Guanine	C5H5N5O	151.1	nucleobase	0.004	0.002	0.1	0.04
TOTAL				55.59	19.58	81.1	33.15

For each metabolite, concentrations are shown for soil WEOC (in mg/L and C ppm), the amount added to SDM1 (mg/L) and the equivalent SDM1 carbon ppm.