FIGURE 4.

Tissue specific expression patterns of BnDGAT1s in B. napus. Roots (R), stems (S), leaves (L) and the third cauline leaves (Ca), of B. napus lines from four groups (A–D, represent group I–IV respectively) (one line was selected from each group) were collected on 28 DAP (days after pollination) and analyzed by RT-PCR. Petals (Pe), pistils (Pi), stamens (St), and sepals (Se) were collected at same stage and analyzed by RT-PCR. Actin was used as internal reference. Primers and semi-quantitative PCR conditions are described in Section “Materials and Methods.” L indicates low seed oil and H indicates high seed oil.