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. Author manuscript; available in PMC: 2017 Dec 27.
Published in final edited form as: Virology. 2015 Aug 1;485:116–127. doi: 10.1016/j.virol.2015.07.008

Fig. 3.

Fig. 3

Extracellular vesicles (EVs) containing recombinant HPgV E2 protein inhibit IL-12-mediated signaling and deliver E2 to NK cells. IFNγ release by the NK cell line (NK92MI) following incubation with CHO cell culture supernatant EVs containing recombinant HPgV E2-human Fc fusion protein or human Fc protein (A). Cells were incubated 18 h prior to stimulation with IL-12 (p value for T test shown). E2-Fc and Fc concentration used was 50 µg/ml. EVs from cell culture supernatant fluids of cells expressing HPgV E2-Fc contained E2, while EVs from CHO cells expressing just Fc protein did not (B). NK92MI cells were incubated overnight with HPgV E2 positive EVs and the cells examined for surface or total cellular E2 protein following permeabilization using flow cytometry (C). The mean fluorescence intensity (MFI) of surface and total HPgV E2 in the cells is summarized in the graph (C).