Table 2.
Distribution of RNAi-associated genes in the kinetoplastid genome
| Strain | Host | Argonaute | Dicer1 | Dicer2 | |||
|---|---|---|---|---|---|---|---|
|
|
|
|
|||||
| Aa | Expect | Aa | Expect | Aa | Expect | ||
| Bodo sp. | Free-living | Part | 6e-19 | Part | 2e-12 | N.F | |
| P. caudatus | Free-living | N.F | N.F | N.F | |||
| L. major | Human | N.F | N.F | N.F | |||
| L. braziliensis | Human | 898 | 0.0 | 2214 | 0.0 | 1330 | 0.0 |
| T. cruzi G | Opossum | N.F | N.F | N.F | |||
| T. brucei brucei | Diptera | 903 | 1e-110 | 1648 | 2e-76 | 948 | 1e-11 |
| T. b. gambiense | Human | 914 | 1e-103 | 1648 | 1e-76 | 946 | 8e-12 |
| T. cruzi 1994 | Bat | N.F | N.F | N.F | |||
| T. cruzi CL | Hemiptera | N.F | N.F | N.F | |||
| T.cruzi marinkellei | Bat | N.F | N.F | N.F | |||
| T.dionisii | Bat | N.F | N.F | N.F | |||
| T. erneyi | Bat | N.F | N.F | N.F | |||
| T. rangeli AM80 | Human | 873 | 1e-100 | 1642 | 5e-71 | 941 | 3e-11 |
| T. conorhini | Rat | 905 | 1e-102 | 1686 | 8e-74 | 937 | 1e-11 |
| T. lewisi | Rat | N.F | N.F | N.F | |||
| E. schaudinni | Sloth | N.F | N.F | N.F | |||
| C. fasciculata | Diptera | 965 | 0.0 | 2240 | 0.0 | 1251 | 0.0 |
| C. acanthocephali | Hemiptera | 931 | 0.0 | 2201 | 0.0 | 1499 | 0.0 |
| L. costaricensis BR | Hemiptera | 959 | 0.0 | 2379 | 0.0 | 1316 | 0.0 |
| H. muscarum | Diptera | 915 | 1e-144 | 2000 | 6e-84 | 1380 | 8e-14 |
| Phytomonas sp. Jma | Plant | N.F | N.F | N.F | |||
| A. deanei | Hemiptera | 882 | 1e-114 | 1412 | 9e-71 | N.F | |
| A. desouzai | Diptera | 893 | 1e-120 | 1336 | 3e-81 | N.F | |
| S. galati | Diptera | N.F | N.F | N.F | |||
| S. oncopelti | Hemiptera | N.F | N.F | N.F | |||
| S. culicis | Diptera | N.F | N.F | N.F | |||
Sequences of AGO1, DCL1 and DCL2 genes identified in Leishmania braziliensis and C. fasciculata genomes (Lye et al. 2010) were used as queries in searches of similarities; BLAST p values were based on amino acid sequences from L. braziliensis. In addition, T. brucei brucei TREU927, T. b. gambiense DAL972 and L. major Friedlin were used as reference strains (see Table 1)
Hosts are specified by the original source of organisms; however, some organisms, like T. cruzi strains G and CL, or, strains of Leishmania are pathogenic to humans. The organism of isolation for C. fasciculata, was likely a mosquito
This table includes incomplete data on Bodo sp. ATCC50149 proteins AGO1 and DCL1, whose genes have been identified in the genome. Due to the scarcity of the available material, it was not possible to obtain amino acid sequences that would form complete ORFs with presumably only small portions missing. It was found important though to include this organism, being the only free-living kinetoplastid with identified RNAi genes among those tested, in this work
Aa number of amino acids in the protein, Part partial open reading frame, NF not found