Fig. 1. Effects of caudal fourth ventricular (CV4) administration of the adenosine 5′-monophosphate-activated protein kinase inhibitor compound C (Cc) on refeeding by estradiol (E)-treated ovariectomized (OVX) female rats after variable-length food deprivation (FD). Animals were exposed to FD (food was withdrawn at 9:00 PM) over a period of 12 (FD-12) or 18 (FD-18) hours, then injected into the CV4 with the vehicle (V; horizontal bars) or Cc (5.0 µg/2.0 µL DMSO; cross-hatched bars) prior to assessment of rebound feeding over a one hour period. Full-fed (FF; open bars) were treated by intra-CV4 V injection. Bars on the left side of the graph depict mean weight of consumed food±standard error of the mean after FD-12 by groups of 30 (E-30) or 300 (E-300) µg E/mL-implanted OVX rats treated by FF/V, FD/V, or FD/Cc (n=6 rats/group). On the right-hand side of the graph, bars show mean intake±SEM after FD-18 by groups of 30 (E-30) or 300 (E-300) µg E/mL-implanted OVX rats treated by FF/V, FD/V, or FD/Cc (n=6 rats/group). ^aP<0.05 vs. FF; ^bP<0.05 vs. FD+V; ^cP<E-30 FF+V.