Table 2.
Summary of biochemical properties of CAS-2 variants
| Protein | Kd forATP-actin binding (μM) | Kd forADP-actin binding (μM) | Nucleotide exchange activity | Actin recycling in competition with ADF/cofilin |
|---|---|---|---|---|
| MBP | No binding | No binding | - | - |
| MBP-CAS2C (WT) | 0.33 | 0.02 | +++ | +++ |
| MBP-CAS2C (V322A) | 0.41 | 0.03 | +++ | +++ |
| MBP-CAS2C (V367A) | 5.8* | 2.9* | + | + |
| MBP-CAS2C (I373A) | No binding | 19* | + | + |
| MBP-CAS2C (L383A) | 2.3* | 0.48 | +++ | ++ |
| MBP-CAS2C (V411A) | 9.8* | 5.8* | + | + |
| MBP-CAS2C (V367A/I373A) | No binding | No binding | - | - |
| MBP-CAS2 (WT) | ND1 | ND | +++ | +++ |
| MBP-CAS2 (V367A/I373A) | ND | ND | - | - |
1
Not determined.
*
Saturation of binding was not experimentally confirmed. Kd values were calculated by curve fitting assuming a saturation value that is reached by MBP-CAS-2C (WT).