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. 2017 Dec 22;10:432. doi: 10.3389/fnmol.2017.00432

FIGURE 4.

FIGURE 4

PTEN inhibitor alleviated sevoflurane-induced autophagy in the fetal brains. G14 rats were pretreated with bpV 1 h before sevoflurane exposure, expression of p-Akt/Akt and mTOR was upregulated (A,B), and LC3BII and Beclin-1 expression was alleviated with bpV treatment (C). LC3B (green) co-localized with Nestin (red) showed punctate pattern in the 3.5%SEV group, whereas both the CON group and 3.5%SEV + bpV group presented diffused green light labeling (D). Scale bar = 50 μm. Quantification of p-Akt/Akt, mTOR, LC3BII and Beclin-1 (E). Values are presented as mean ± SD, n = 5; ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001 compared with the CON group, #P < 0.05, ##P < 0.01, ###P < 0.001 compared with the 3.5%SEV group. One-way ANOVA with Newman–Keuls post hoc test or Kruskal–Wallis with Dunn’s Multiple comparison test was used for data analysis.